International Journal of Agriculture Extension and Social Development

author(s)
Shakir Ullah, Lubna Begum, Maria Khattak, Robeena Naz, Mohammad Sohil and Mohsin Ihsan

abstract
In the present research work the phytochemical and Chromatographic separation of bio active compounds Berberis lycium occurred. Phytochemical investigation of the CH₂Cl₂/CH₃OH (1:1) extract ethanol of root shown the presence of terpenoids, flavonoids, saponins, anthraquinones and alkaloids, steroids and tannins was absent. Chromatographic separation of CH₂Cl₂/CH₃OH (1:1) root ethanol extract yielded methyl 9, 10-dihydro-3, 8-hydroxy-6-methyl-9, 10-dioxoanthracene-2-carboxylate, isolated for the first time from the species of Berberis lycium. With the help of spectroscopic methods (IR, ¹H NMR, ¹³C NMR, and DEPT-135 and UV-Vis,) Partial characterization of the isolated compound was done.